[2018] [2017] [2016] [2015] [2014] [2013] [2012] [2011] [2010] [2009] [2008] [2007] [2006] [2005] [2004] [2003] [2002] [2001] [2000] [1999] [1998] [1997] [1996] [1996] [1996] [1995] [1994] [1993] [1992] [1991] [1990] [1989] [1988] [1987] [1986] [1985] [1984] [1984] [1983] [1982] [当ラボ学生の学会発表記録]

*いくつかの論文は京都大学学術情報レポジトリKURENAI(紅)で全文を公開しています。[KURENAI]のリンクからアクセスしてください。*

    【2018】

  • 【論文】
    Nitta N, Sugimura T, Isozaki A, Mikami H, Hiraki K, Sakuma S, Iino T, Arai F, Endo T, Fujiwaki Y, Fukuzawa H,Hase M,Hayakawa T, Hiramatsu K,Hoshino Y, Inaba M, Ito T, Karakawa H, Kasai Y, Koizumi K, Lee S, Lei C,Li M, Maeno T, Matsusaka S, Murakami D, Nakagawa A, Oguchi Y, Oikawa M, Ota T, Shiba K, Shintaku H, Shirasaki Y, Suga K, Suzuki Y, Suzuki N, Tanaka Y, Tezuka H, Toyokawa C, Yalikun Y, Yamada M, Yamagishi M, Yamano T, Yasumoto A, Yatomi Y, Yazawa M, Di Carlo D, Hosokawa Y, Uemura S, Ozeki Y, Goda K
    Intelligent Image-Activated Cell Sorting
    Cell in press [DOI]
    東大、JST、理研他との共同研究成果です。今回開発された技術「Intelligent Image-Activated Cell Sorter」により、従来は手作業でおこなっていた希少細胞(変異株)の選抜時間が約6,500倍に短縮され、偶然の幸運な発見(Serendipity)が可能になることを実証しました。私たちは、環境中のCO2濃度を検知して葉緑体内部で移動するタンパク質を可視化し、その局在が異常になった変異株の単離に成功しました。今後は、得られた変異株を調べる事で、光合成炭素固定を支えるCO2濃縮機構の制御や葉緑体内部でのタンパク質移行のメカニズムの解明が期待されます。京大プレス・リリースNature Research Highlights時事通信日刊工業新聞、海外サイト(1)で紹介されました。


  • 【教科書】
    「京大発!フロンティア生命科学」京都大学大学院生命科学研究科 編 講談社 [Amazon Link]
    1章「生き物の由来 地球と生命の進化」(執筆:福澤 秀哉)
    18章「体の設計図を読む ゲノム情報と進化」(執筆:山野 隆志)


  • 【和文総説】
    梶川 昌孝、福澤 秀哉

    「実用珪藻の代謝工学による高付加価値油脂の生産ツノケイソウでのリシノール酸生産と細胞毒性の回避」 化学と生物 56(4):246-247 (2018) [HP Link]
    2016年にSci Repに報告したツノケイソウにおけるリシノール酸生産についての和文総説です。


  • 【論文】
    Yamano T, Toyokawa C, Fukuzawa H

    High-resolution suborganellar localization of Ca2+-binding protein CAS, a novel regulator of CO2-concentrating mechanism.
    Protoplasma 255(4):1015-1022 (2018) [DOI] [KURENAI] [Pubmed]
    Wang et al. 2016 PNASで報告したCa2+結合タンパク質CASの細胞内局在を高解像度イメージング技術で明らかにしました。Editorialで紹介されました。


    [2017]

  • 【論文】
    Bowman JL, Kohchi T, Yamato KT, Jenkins J, Shu S, Ishizaki K, Yamaoka S, Nishihama R, Nakamura Y, Berger F, Adam C, Aki SS, Althoff F, Araki T, Arteaga-Vazquez MA, Balasubrmanian S, Barry K, Bauer D, Boehm CR, Briginshaw L, Caballero-Perez J, Catarino B, Chen F, Chiyoda S, Chovatia M, Davies KM, Delmans M, Demura T, Dierschke T, Dolan L, Dorantes-Acosta AE, Eklund DM, Florent SN, Flores-Sandoval E, Fujiyama A, Fukuzawa H, Galik B, Grimanelli D, Grimwood J, Grossniklaus U, Hamada T, Haseloff J, Hetherington AJ, Higo A, Hirakawa Y, Hundley HN, Ikeda Y, Inoue K, Inoue SI, Ishida S, Jia Q, Kakita M, Kanazawa T, Kawai Y, Kawashima T, Kennedy M, Kinose K, Kinoshita T, Kohara Y, Koide E, Komatsu K, Kopischke S, Kubo M, Kyozuka J, Lagercrantz U, Lin SS, Lindquist E, Lipzen AM, Lu CW, De Luna E, Martienssen RA, Minamino N, Mizutani M, Mizutani M, Mochizuki N, Monte I, Mosher R, Nagasaki H, Nakagami H, Naramoto S, Nishitani K, Ohtani M, Okamoto T, Okumura M, Phillips J, Pollak B, Reinders A, Rovekamp M, Sano R, Sawa S, Schmid MW, Shirakawa M, Solano R, Spunde A, Suetsugu N, Sugano S, Sugiyama A, Sun R, Suzuki Y, Takenaka M, Takezawa D, Tomogane H, Tsuzuki M, Ueda T, Umeda M, Ward JM, Watanabe Y, Yazaki K, Yokoyama R, Yoshitake Y, Yotsui I, Zachgo S, Schmutz J.
    Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome.
    Cell 171(2):287-304 (2017) [DOI] [KURENAI] [Pubmed]
    詳細はこちらを参照してください。
  • 【和文総説】
    福澤 秀哉、梶川 昌孝

    「実用珪藻ツノケイソウによるリシノール酸の生産」 バイオイエンスとインダストリー 75(3):242-243 (2017) [書誌リンク]
    2016年にSci Repに報告したツノケイソウにおけるリシノール酸生産についての和文総説です。
  • 【論文】
    Kinoshita A, Niwa Y, Onai K, Yamano T, Fukuzawa H, Ishiura M, Matsuo T
    CSL encodes a leucine-rich-repeat protein implicated in red/violet light signaling to the circadian clock in Chlamydomonas.
    PLOS Genetics 13(3):e1006645 (2017) [DOI] [KURENAI] [Pubmed]
    我々の遺伝子リソースを用いた共同研究です。名古屋大学HP京都大学HP、海外のサイト(1, 2)でも紹介されました。
  • 【論文】
    Tsuji T, Kurokawa Y, Chiche J, Pouyssegur J, Sato H, Fukuzawa H, Nagao M, and Kambe T
    Dissecting the process of activation of cancer-promoting zinc-requiring ectoenzymes by zinc metalation mediated by ZnT transporters.
    Journal of Biological Chemistry 292(6):2159-2173 (2017) [DOI] [Pubmed]
    [2016]

  • 【和文総説】
    福澤 秀哉

    「CO2資源化の効率を左右するCO2濃縮機構とは」
    化学と工業 69(11):960-962 (2016) [PDF](許可を受けて掲載しています)
    最新の成果を元にしたCO2濃縮のレビューと今後の展望を書きました。
  • 【論文】
    Kajikawa M, Abe T,
    Ifuku K, Furutani K, Yan D, Okuda T, Ando A, Kishino S, Ogawa J, Fukuzawa H
    Production of ricinoleic acid-containing monoestolide triacylglycerides in an oleaginous diatom, Chaetoceros gracilis.
    Scientific Reports 6:36809 (2016) [DOI] [KURENAI] [Pubmed]
    牡蠣などの養殖で利用される実用珪藻のツノケイソウで、医薬品や化成品原料となるリシノール酸の生産に成功しました。微細藻類を用いた有用物質生産に繋がる成果です。[京都大学HP] [日経バイオテク] [日刊工業新聞誌面] [日刊工業新聞Web版]で紹介されました。
  • 【論文】
    Wang L, Yamano T, Takane T, Niikawa Y, Toyokawa C,
    Ozawa S, Tokutsu R, Takahashi Y, Minagawa J, Kanesaki Y, Yoshikawa H, Fukuzawa H
    Chloroplast-mediated regulation of CO2-concentrating mechanism by Ca2+-binding protein CAS in the green alga Chlamydomonas reinhardtii.
    Proc. Natl. Acad. Sci. USA 113(44):12586-12591 (2016) [DOI] [KURENAI] [Pubmed]
    我々が昨年度に発見した重炭酸イオン輸送体が、植物に特異的なカルシウム結合タンパク質CASによって葉緑体を介して制御されることを明らかにしました。また、CASがCO2や光に依存して葉緑体の中を動くといった、今後の展開が楽しみな知見も得られました。
    [京都大学HP] [国立環境研ニュースサイト]で紹介されました。
  • 【和文総説】
    山野 隆志、福澤 秀哉

    「微細藻の光合成を支える重炭酸イオン輸送 ー細胞膜と葉緑体包膜に局在する重炭酸イオン輸送体の発見ー」
    化学と生物 54(7):459-460. (2016) [HP Link]
    2015年にPNAS誌に報告した内容の解説と、輸送体研究の今後の展望を書きました。


  • 【プロトコル】
    Yamano T, Fukuzawa H

    Indirect Immunofluorescence Assay in Chlamydomonas reinhardtii.
    Bio-protocol: 6(13) e1864 (2016)
    細胞内におけるタンパク質の局在を調べるときに使われる間接的免疫蛍光染色法について、その詳細なプロトコルを紹介したものです。
  • 【特許】
    Fukuzawa H, Yamano T
    , Ifuku K, Hayakawa Y
    発明の名称「Method of transferring gene into algal cell involving utilizing multiple square-wave pulses in three steps」米国特許 番号9255276
    2013年にJ Biosci Bioeng誌に報告した内容を含む形質転換技術の米国特許が成立しました。特許証はこちら
    [2015]

  • 【論文】
    Yamano T, Sato E, Iguchi H, Fukuda Y, Fukuzawa H

    Characterization of cooperative bicarbonate uptake into chloroplast stroma in the green alga Chlamydomonas reinhardtii.
    Proc. Natl. Acad. Sci. USA 112(23):7315-7320 (2015) [DOI] [KURENAI] [Pubmed]
    藻類が水中のCO2欠乏環境でも光合成を維持して生存するために、細胞内にCO2濃縮することは今から35年前に発見されましたが、その輸送を担っている分子の詳細は不明でした。今回、単細胞緑藻クラミドモナスをモデルとして、細胞膜に局在するHLA3と葉緑体包膜に局在するLCIAが協調して、重炭酸イオン(bicarbonate)を細胞の外から葉緑体内部(ストロマ)に輸送・濃縮することで、光合成を維持していることを明らかにしました。
    [京都大学HP] [京都新聞Web版] [京都新聞夕刊] [国立環境研ニュースサイト] [月刊化学7月号] [サイエンスポータル]で紹介されました。
  • 【和文総説】
    福澤 秀哉

    「二酸化炭素の固定と濃縮」光合成のエネルギー変換と物質変換(杉浦 美羽他 編)pp.202-214 (2015) [HP Link]
  • 【論文】
    Kajikawa M, Sawaragi Y, Shinkawa H, Yamano T,
    Ando A, Kato M, Hirono M, Sato N, Fukuzawa H
    Algal dual-specificity tyrosine-phosphorylation-regulated kinase TAR1 regulates accumulation of triacylglycerol in nitrogen- or sulfur-deficiency.
    Plant Physiology 168(2):752-764 (2015) [DOI] [Pubmed]
    多くの藻類では硫黄や窒素欠乏条件下でトリアシルグリセロール(TAG)の蓄積が誘導されます。緑藻クラミドモナスのTAG蓄積異常変異体tar1-1の解析からその制御機構を担うリン酸化酵素遺伝子TAR1を同定しました。TAR1は硫黄と窒素欠乏に共通して培地中の酢酸を元にしたTAGの蓄積を正に制御し、窒素欠乏下の光合成活性の抑制にも関与することが分かってきました。
  • 【特許】
    福澤 秀哉、山野 隆志、
    伊福 健太郎、早川 靖彦
    発明の名称「3段階方式矩形波多重パルスを利用した藻類細胞への遺伝子導入法」国内特許:第5721191号
    2013年にJ Biosci Bioeng誌に報告した内容を含む形質転換技術の国内特許が成立しました。特許証はこちら
  • 【論文】
    Kajikawa M, Kinohira S,
    Ando A, Shimoyama M, Kato M, Fukuzawa H
    Accumulation of squalene in a microalga Chlamydomonas reinhardtii by genetic modification of squalene synthase and squalene epoxidase genes.
    PLOS ONE 10(3):e0120446 (2015) [DOI] [Pubmed]
    緑藻クラミドモナスのステロール生合成経路の酵素遺伝子を改変することにより、化粧品基材、医薬品、栄養サプリメントなど様々な分野で利用可能な有用物質スクアレンを蓄積する株を創出しました。
    [2014]

  • 【論文】
    Kihara H, Tanaka M, Yamato TK, Horibata A, Yamada A, Kita S, Ishizaki K, Kajikawa M, Fukuzawa H, Kohchi T, Akakabe Y, Matsui K
    Arachidonic acid-dependent carbon-eight volatile synthesis from wounded liverwort (Marchantia polymorpha).
    Phytochemistry 107:42-49 (2014) [DOI] [Pubmed]
    ゼニゴケを用いた共同研究です。
  • 【論文】
    Wang L, Yamano T, Kajikawa M,
    Hirono M, Fukuzawa H
    Isolation and characterization of novel high-CO2 requiring mutants of Chlamydomonas reinhardtii.
    Photosynthesis Research 121(2-3):175-184 (2014) [DOI] [KURENAI] [Pubmed]
    DNAタギングにより得られた約20,000株の形質転換株について、高CO2条件と低CO2条件での生育を比較し、LC条件でのみ生育が顕著に遅延する3株の高CO2要求性変異株を単離・解析しました。これらの変異株をさらに解析することで、クラミドモナスのCO2シグナル伝達の解明に向けて重要な知見を得ることができると考えています。
  • 【論文】
    Yamano T, Asada A, Sato E, Fukuzawa H

    Isolation and characterization of mutants defective in the localization of LCIB, an essential factor for the carbon-concentrating mechanism in Chlamydomonas reinhardtii.
    Photosynthesis Research 121(2-3):193-200 (2014) [DOI] [KURENAI] [Pubmed]
    クラミドモナスのCO2濃縮に必須なLCIBタンパク質は、光の明暗とCO2濃度の変化によって葉緑体内で局在を変化させることが分かっています(Yamano et al. 2010 Plant Cell Physiol.)。この論文ではその局在変化のメカニズムに迫る足がかりとして、DNAタギングにより得られた約13,000株の形質転換株について1株ずつLCIB局在の蛍光観察を行い、12株のLCIB局在異常変異株(aberrant LCIB localization, abl株)を単離・解析しました。その中には、CO2濃縮において重要な炭酸固定の場であるピレノイドの細胞内での位置や数が異常になった変異株が多く含まれており、LCIBとピレノイドの密接な関係が明らかになってきました。
    [2013]

  • 【論文】
    Yamano T, Iguchi H, Fukuzawa H

    Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal.
    Journal of Bioscience and Bioengineering 115:691-694 (2013) [DOI] [KURENAI] [Pubmed]
    3段階方式矩形波多重パルスによるエレクトロポレーション法を利用した、迅速かつ高効率のクラミドモナス形質転換系を開発しました。従来の方法では、クラミドモナスの細胞壁を溶解させる必要があり、この溶解酵素の単離・溶解反応などのステップに多くの時間を要していました。今回報告した方法では、細胞壁を溶解する必要はなく、細胞とDNAを直接混ぜた後にエレクトロポレーションによる形質転換が可能となりました。これによって、大規模な遺伝子破壊株ライブラリーの作成などが格段に容易になり、PCRスクリーニングによって調べたい遺伝子の変異株を単離することが可能となりました。この技術はYamano et al. Photosynth Res. (2014), Wang et al. Photosynth Res. (2014), Kajikawa et al. Plant Phys. (2015), Yamano et al. PNAS (2015)で報告した変異株単離において利用されています。

  • 【和文総説】
    福澤秀哉

    藻類のCO2濃縮作用と光合成維持の機構光合成研究-微細藻類の大量生産・事業化に向けた培養技術
    情報機構 pp.212-218 (2013) [Link]
    [2012]

  • 【論文】
    福澤秀哉、山野隆志、梶川昌孝

    「緑藻クラミドモナスにおける無機炭素濃縮機構と脂質代謝」
    光合成研究 22(3) [KURENAI]
  • 【和文総説】
    山野隆志、福澤秀哉

    第4章 「微細藻類のCO2濃縮機構 -モデル緑藻におけるゲノム発現情報の利用-」微細藻類によるエネルギー生産と事業展望
    シーエムシー出版 [Link]
  • 【和文総説】
    山野隆志、福澤秀哉

    1-2-1-11「藻類の核酸(DNAとRNA)- 遺伝子の特徴と解析法」
    藻類ハンドブック エヌ・ティー・エス出版 [Link]
  • 【総説】
    Hideya Fukuzawa, Teruo Ogawa, and Aaron Kaplan

    The Uptake of CO2 by Cyanobacteria and Microalgae.
    In Photosynthesis "Plastid Biology, Energy Conversion and Carbon Assimilation" (Ed. by Eaton-rye J.J., Tripathy B. C., and Sharkey T.D.) Springer, Advances in Photosynthesis and Respiration 34: 625-650 [PDF]
    [2011]

  • 【論文】
    Yamano T, Fujita A, Fukuzawa H

    Photosynthetic characteristics of a multicellular green alga Volvox carteri in response to external CO2 levels possibly regulated by CCM1/CIA5 ortholog.
    Photosynthesis Research 109:151-159 [pubmed]
  • 【論文】
    Matsuo M, Hachisu R, Tabata S, Fukuzawa H, Obokata J

    Transcriptome Analysis of Respiration-Responsive Genes in Chlamydomonas reinhardtii: Mitochondrial Retrograde Signaling Coordinates the Genes for Cell Proliferation with Energy-Producing Metabolism.
    Plant Cell Physiol. 52:333-43 [pubmed]
    [2010]

  • 福澤秀哉
    「微細藻類におけるCO2濃縮機構と物質生産」 学術の動向 15(12):70-71 (2010) [PDF]

  • Ohnishi N, Mukherjee B, Tsujikawa T, Yanase M, Nakano H, Moroney JV, Fukuzawa H
    Expression of a low-CO2-inducible protein, LCI1, increases inorganic carbon uptake in the green alga Chlamydomonas reinhardtii. Plant Cell 22:3105-3117 (2010) [pubmed]
  • Yamano T, Tsujikawa T, Hatano K, Ozawa S, Takahashi Y, Fukuzawa H
    Light and low-CO2 dependent LCIB/LCIC complex localization in the chloroplast supports the carbon-concentrating mechanism in Chlamydomonas reinhardtii. Plant Cell Physiol. 51:1453-1468 (2010) [pubmed]
    [2009]

  • Yamano T and Fukuzawa H
    Carbon-concentrating mechanism in a green alga, Chlamydomonas reinhardtii, revealed by transcriptome analyses. J Basic Microbiol. 49, 42-51 (2009) [pubmed]
  • Kubo T, Kaida S, Abe J, Saito T, Fukuzawa H, Matsuda Y
    The Chlamydomonas hatching enzyme, sporangin, is expressed in specific phases of the cell cycle and is localized to the flagella of daughter cells within the sporangial cell wall. Plant Cell Physiol. 50:572-583 (2009) [pubmed]
  • Uchida H, Yamashita H, Kajikawa M, Ohyama, Nakayachi O, Sugiyama R, Yamato KT, Muranaka T, Fukuzawa H, Takemura M, Ohyama K
    Cloning and characterization of a squalene synthase gene from a petroleum plant, Euphorbia tirucalli L. Planta 229:1243-1252 (2009) [pubmed]
    [2008]

  • 福澤秀哉、久保雄昭、山野隆志
    「緑藻クラミドモナスのゲノムから植物と動物の機能を探る」 蛋白質核酸酵素 53:1133-43 (2008) [全文PDF]

    訂正【本文(p.1138,右段3〜4行目)】
    [誤] ・・・炭酸脱水素酵素をコードする遺伝子が含まれている.また,細胞表層炭酸脱水素酵素の遺伝子は・・・
    [正] ・・・炭酸脱水酵素をコードする遺伝子が含まれている.また,細胞表層炭酸脱水酵素の遺伝子は・・・

  • Yamano T, Miura K, Fukuzawa H
    Expression analysis of genes associated with the induction of the carbon-concentrating mechanism in Chlamydomonas reinhardtii. Plant Physiol. 147:340-54 (2008) [pubmed]
  • Kohinata T, Nishino H, Fukuzawa H
    Significance of zinc in a regulatory protein, CCM1, which regulates the carbon-concentrating mechanism in Chlamydomonas reinhardtii. Plant Cell Physiol. 49:273-83 (2008) [pubmed]
  • Kubo T, Abe J, Oyamada T, Ohnishi M, Fukuzawa H, Matsuda Y, Saito T
    Characterization of novel genes induced by sexual adhesion and gamete fusion and of their transcriptional regulation in Chlamydomonas reinhardtii. Plant Cell Physiol. 49:981-993 (2008) [pubmed]
  • Kajikawa M, Matsui K, Ochiai M, Tanaka Y, Kita Y, Ishimoto M, Kohzu Y, Shoji S, Yamato KT, Ohyama K, Fukuzawa H, Kohchi T
    Production of arachidonic and eicosapentaenoic acids in plants using bryophyte fatty acid delta6-desaturase, delta6-elongase, and delta5-desaturase genes. Biosci. Biotechnol. Biochem. 72:435-444 (2008) [pubmed] <日本農芸化学会2008年論文賞受賞>
  • Fathinejad S, Steiner JM, Reipert S, Marchetti M, Allmaier G, Burey SC, Ohnishi N, Fukuzawa H, Loffelhardt W, Bohnert HJ
    A carboxysomal carbon-concentrating mechanism in the cyanelles of the 'coelacanth' of the algal world, Cyanophora paradoxa? Physiol Plant. 133:27-32 (2008) [pubmed]
    [2007]

  • 福澤秀哉、山野隆志
    「植物の比較ゲノム:緑藻のゲノムからみた光合成生物の進化と多様性」 細胞工学 9月25日発行 (2007)
  • Merchant SS, Prochnik SE, Vallon O, Harris EH, Karpowicz SJ, Witman GB, Terry A, Salamov A, Fritz-Laylin LK, Marechal-Drouard L, Marshall WF, Qu LH, Nelson DR, Sanderfoot AA, Spalding MH, Kapitonov VV, Ren Q, Ferris P, Lindquist E, Shapiro H, Lucas SM, Grimwood J, Schmutz J, Cardol P, Cerutti H, Chanfreau G, Chen CL, Cognat V, Croft MT, Dent R, Dutcher S, Fernandez E, Fukuzawa H, Gonzalez-Ballester D, Gonzalez-Halphen D, Hallmann A, Hanikenne M, Hippler M, Inwood W, Jabbari K, Kalanon M, Kuras R, Lefebvre PA, Lemaire SD, Lobanov AV, Lohr M, Manuell A, Meier I, Mets L, Mittag M, Mittelmeier T, Moroney JV, Moseley J, Napoli C, Nedelcu AM, Niyogi K, Novoselov SV, Paulsen IT, Pazour G, Purton S, Ral JP, Riano-Pachon DM, Riekhof W, Rymarquis L, Schroda M, Stern D, Umen J, Willows R, Wilson N, Zimmer SL, Allmer J, Balk J, Bisova K, Chen CJ, Elias M, Gendler K, Hauser C, Lamb MR, Ledford H, Long JC, Minagawa J, Page MD, Pan J, Pootakham W, Roje S, Rose A, Stahlberg E, Terauchi AM, Yang P, Ball S, Bowler C, Dieckmann CL, Gladyshev VN, Green P, Jorgensen R, Mayfield S, Mueller-Roeber B, Rajamani S, Sayre RT, Brokstein P, Dubchak I, Goodstein D, Hornick L, Huang YW, Jhaveri J, Luo Y, Martinez D, Ngau WC, Otillar B, Poliakov A, Porter A, Szajkowski L, Werner G, Zhou K, Grigoriev IV, Rokhsar DS, Grossman AR
    The Chlamydomonas genome reveals evolutionary insights into key animal and plant functions. Science 318:245-250 (2007) [pubmed]
  • Hanawa Y, Watanabe M, Karatsu Y, Fukuzawa H, Shiraiwa Y
    Induction of a high-CO2-inducible, periplasmic protein, H43, and its application as a high-CO2-responsive marker for study of the high-CO2-sensing mechanism in Chlamydomonas reinhardtii. Plant Cell Physiol. 48:299-309 (2007) [pubmed]
  • Chiyoda S, Linley PJ, Yamato KT, Fukuzawa H, Yokota A, Kohchi T
    Simple and efficient plastid transformation system for the liverwort Marchantia polymorpha L. suspension-culture cells. Transgenic Res. 16:41-49 (2007) [pubmed]
  • Yamato KT, Ishizaki K, Fujisawa M, Okada S, Nakayama S, Fujishita M, Bando H, Yodoya K, Hayashi K, Bando T, Hasumi A, Nishio T, Sakata R, Yamamoto M, Yamaki A, Kajikawa M, Yamano T, Nishide T, Choi SH, Shimizu-Ueda Y, Hanajiri T, Sakaida M, Kohno K, Takenaka M, Yamaoka S, Kuriyama C, Kohzu Y, Nishida H, Brennicke A, Shin-i T, Kohara Y, Kohchi T, Fukuzawa H, Ohyama K
    Gene organization of the liverwort Y chromosome reveals distinct sex chromosome evolution in a haploid system. Proc. Natl. Acad. Sci. USA 104:6472-6477 (2007) [pubmed]
  • Burey SC, Poroyko V, Ergen ZN, Fathi-Nejad S, Schuller C, Ohnishi N, Fukuzawa H, Bohnert HJ, Loffelhardt W
    Loelhardt Acclimation to low CO2 by an inorganic carbon-concentrating mechanism in Cyanophora paradoxa. Plant Cell & Environment 30:1422-1435 (2007) [pubmed]
  • Ozaki H, Ikeuchi M, Ogawa T, Fukuzawa H, Sonoike K
    Large-scale analysis of chlorophyll fluorescence kinetics in Synechocystis sp. PCC 6803: identification of the factors involved in the modulation of photosystem stoichiometry. Plant Cell Physiol. 48:451-8 (2007) [pubmed]
    [2006]

  • Kajikawa M, Yamato KT, Kohzu Y, Shoji S, Matsui K, Tanaka Y, Sakai Y, Fukuzawa H
    A front-end desaturase from Chlamydomonas reinhardtii produces pinolenic and coniferonic acids by omega13 desaturation in methylotrophic yeast and tobacco. Plant Cell Physiol. 47:64-73 (2006) [pubmed]
  • Kajikawa M, Yamato KT, Sakai Y, Fukuzawa H, Ohyama K, Kohchi T
    Isolation and functional characterization of fatty acid delta5-elongase gene from the liverwort Marchantia polymorpha L. FEBS Lett. 580:149-154 (2006) [pubmed]
    [2005]

  • 福澤秀哉、山野隆志
    「二酸化炭素による転写調節機構 − 緑藻クラミドモナスのCO2濃縮機構」 蛋白質核酸酵素 50:958-965 (2005) [全文PDF]
  • Kucho K, Okamoto K, Tabata S, Fukuzawa H, Ishiura M
    Identification of novel clock-controlled genes by cDNA macroarray analysis in Chlamydomonas reinhardtii. Plant Mol Biol. 57:889-906 (2005) [pubmed]
  • Kajikawa M, Yamato KT, Fukuzawa H, Sakai Y, Uchida H, Ohyama K
    Cloning and characterization of a cDNA encoding beta-amyrin synthase from petroleum plant Euphorbia tirucalli L. Phytochemistry 66:1759-66 (2005) [pubmed]
  • Uchida H, Isono K, Tomizawa K, Iwano M, Yamashita H, Fukuzawa H, Ohyama K, Yokota A
    Synthesis of Rubisco gene products is upregulated by increasing the copy number of rbcL gene in Chlamydomonas chloroplast genome, without increased accumulation of the two Rubisco subunits. Plant Biotech. 22:145-149 (2005)
    [2004]

  • Yoshioka S, Taniguchi F, Miura K, Inoue T, Yamano T, Fukuzawa H
    The novel Myb transcription factor LCR1 regulates the CO2-responsive gene Cah1, encoding a periplasmic carbonic anhydrase in Chlamydomonas reinhardtii. Plant Cell 16:1466-77 (2004) [pubmed]
  • Miura K, Yamano T, Yoshioka S, Kohinata T, Inoue Y, Taniguchi F, Asamizu E, Nakamura Y, Tabata S, Yamato KT, Ohyama K, Fukuzawa H
    Expression profiling-based identification of CO2-responsive genes regulated by CCM1 controlling a carbon-concentrating mechanism in Chlamydomonas reinhardtii. Plant Physiol. 135:1595-607 (2004) [pubmed]
  • Asamizu E, Nakamura Y, Miura K, Fukuzawa H, Fujiwara S, Hirono M, Iwamoto K, Matsuda Y, Minagawa J, Shimogawara K, Takahashi Y and Tabata S
    Establishment of publicly available cDNA material and information resource of Chlamydomonas reinhardtii (Chlorophyta) to facilitate gene function analysis. Phycologia 43:722-726 (2004) [Journal]
  • Abe J, Kubo T, Takagi Y, Saito T, Miura K, Fukuzawa H, Matsuda Y
    The transcriptional program of synchronous gametogenesis in Chlamydomonas reinhardtii. Curr Genet. 46:304-15 (2004) [pubmed]
  • Kajikawa M, Yamato KT, Kohzu Y, Nojiri M, Sakuradani E, Shimizu S, Sakai Y, Fukuzawa H, Ohyama K
    Isolation and characterization of delta(6)-desaturase, an ELO-like enzyme and delta(5)-desaturase from the liverwort Marchantia polymorpha and production of arachidonic and eicosapentaenoic acids in the methylotrophic yeast Pichia pastoris. Plant Mol Biol. 54:335-52 (2004) [pubmed]
  • Yamaoka S, Takenaka M, Hanajiri T, Shimizu-Ueda Y, Nishida H, Yamato TK, Fukuzawa H and Ohyama K
    A mutant with constitutive sexual organ development in Marchantia polymorpha. Sexual Plant Reproduction 16:253-257 (2004) [Journal]
  • Asadulghani, Nitta K, Kaneko Y, Kojima K, Fukuzawa H, Kosaka H, Nakamoto H
    Comparative analysis of the hspA mutant and wild-type Synechocystis sp. strain PCC 6803 under salt stress: evaluation of the role of hspA in salt-stress management. Arch Microbiol. 182:487-97 (2004) [pubmed]
  • Kajikawa M, Yamato TK, Kohzu Y, Sakata R, Fukuzawa H, Uchida H, Ohyama K
    Expressed sequence tags from callus of Euphorbia tirucalli: A resource for genes involved in triterpenoid and sterol biosynthesis. Plant Biotech. 21:349-353 (2004)
    [2003]

  • Nakazato E, Fukuzawa H, Tabata S, Takahashi H, Tanaka K
    Identification and expression analysis of cDNA encoding a chloroplast recombination protein REC1, the chloroplast RecA homologue in Chlamydomonas reinhardtii. Biosci Biotechnol Biochem. 67:2608-13 (2003) [pubmed]
  • Kucho K, Yoshioka S, Taniguchi F, Ohyama K, Fukuzawa H
    Cis-acting elements and DNA-binding proteins involved in CO2-responsive transcriptional activation of Cah1 encoding a periplasmic carbonic anhydrase in Chlamydomonas reinhardtii. Plant Physiol. 133:783-93 (2003) [pubmed]
  • Suzuki T, Yamasaki K, Fujita S, Oda K, Iseki M, Yoshida K, Watanabe M, Daiyasu H, Toh H, Asamizu E, Tabata S, Miura K, Fukuzawa H, Nakamura S, Takahashi T
    Archaeal-type rhodopsins in Chlamydomonas: model structure and intracellular localization. Biochem Biophys Res Commun. 301:711-7 (2003) [pubmed]
  • Fujisawa M, Nakayama S, Nishio T, Fujishita M, Hayashi K, Ishizaki K, Kajikawa M, Yamato TK, Fukuzawa H, Ohyama K
    Evolution of ribosomal DNA unit on the X chromosome independent of autosomal units in the liverwort Marchantia polymorpha. Chromosome Res. 11:695-703 (2003)
  • Kajikawa M, Yamato KT, Kanamaru H, Sakuradani E, Shimizu S, Fukuzawa H, Sakai Y, Ohyama K
    MpFAE3, a beta-ketoacyl-CoA synthase gene in the liverwort Marchantia polymorpha L., is preferentially involved in elongation of palmitic acid to stearic acid. Biosci Biotechnol Biochem. 67:1667-74 (2003) [pubmed]
  • Kajikawa M, Yamaoka S, Yamato KT, Kanamaru H, Sakuradani E, Shimizu S, Fukuzawa H, Ohyama K
    Functional analysis of a beta-ketoacyl-CoA synthase gene, MpFAE2, by gene silencing in the liverwort Marchantia polymorpha L. Biosci Biotechnol Biochem. 67:605-12 (2003) [pubmed]
    [2002]

  • Kasahara M, Swartz TE, Olney MA, Onodera A, Mochizuki N, Fukuzawa H, Asamizu E, Tabata S, Kanegae H, Takano M, Christie JM, Nagatani A, Briggs WR
    Photochemical properties of the flavin mononucleotide-binding domains of the phototropins from Arabidopsis, rice, and Chlamydomonas reinhardtii. Plant Physiol. 129:762-73 (2002) [pubmed]
  • Miura K, Kohinata T, Yoshioka S, Ohyama K, Fukuzawa H
    Regulation of a carbon concentrating mechanism through CCM1 in Chlamydomonas reinhardtii. Functional Plant Biol. 29:211-219 (2002)
  • Shibata M, Katoh H, Sonoda M, Ohkawa H, Shimoyama M, Fukuzawa H, Kaplan A, Ogawa T
    Genes essential to sodium-dependent bicarbonate transport in cyanobacteria: function and phylogenetic analysis. J Biol Chem. 277:18658-64 (2002) [pubmed]
  • Ishizaki K, Shimizu-Ueda Y, Okada S, Yamamoto M, Fujisawa M, Yamato TK, Fukuzawa H, Ohyama K
    Multicopy genes uniquely amplified in the Y-chromosome-specific repeats of the liverwort, Marchantia polymorpha. Nucleic Acids Res. 30: 4675-4681 (2002)
    [2001]

  • Fukuzawa H, Miura K, Ishizaki K, Kucho KI, Saito T, Kohinata T, Ohyama K
    Ccm1, a regulatory gene controlling the induction of a carbon concentrating mechanism in Chlamydomonas reinhardtii, by sensing CO2 availability. Proc. Natl. Acad. Sci. USA. 98:5347-5352 (2001) [pubmed]
  • Mamedov TG, Suzuki K, Miura K, Kucho Ki K, Fukuzawa H
    Characteristics and sequence of phosphoglycolate phosphatase from a eukaryotic green alga Chlamydomonas reinhardtii. J Biol Chem. 276:45573-9 (2001) [pubmed]
  • Kubo T, Saito T, Fukuzawa H, Matsuda Y
    Two tandemly-located matrix metalloprotease genes with different expression patterns in the chlamydomonas sexual cell cycle. Curr Genet. 40:136-43 (2001) [pubmed]
  • Shibata M, Ohkawa H, Kaneko T, Fukuzawa H, Tabata S, Kaplan A, Ogawa T
    Distinct constitutive and low-CO2-induced CO2 uptake systems in cyanobacteria: genes involved and their phylogenetic relationship with homologous genes in other organisms. Proc Natl Acad Sci U S A. 98:11789-94 (2001) [pubmed]
  • Okada S, Sone T, Fujisawa M, Nakayama S, Takenaka M, Ishizaki K, Kono K, Shimizu-Ueda Y, Hanajiri T, Yamato KT, Fukuzawa H, Brennicke A, Ohyama K
    The Y chromosome in the liverwort Marchantia polymorpha has accumulated unique repeat sequences harboring a male-specific gene. Proc. Natl. Acad. Sci. USA 98:9454-9459 (2001)
  • Fujisawa M, Hayashi K, Nishio T, Bando T, Okada S, Yamato TK, Fukuzawa H, Ohyama K
    Isolation of X- and Y-chromosome-specific DNA markers from a liverwort, Marchantia polymorpha, by representational difference analysis. Genetics 159:981-985 (2001)
    [2000]

  • Asamizu E, Miura K, Kucho K, Inoue Y, Fukuzawa H, Ohyama K, Nakamura Y, Tabata S
    Generation of expressed sequence tags from low-CO2 and high-CO2 adapted cells of Chlamydomonas reinhardtii. DNA Res. 7:305-7 (2000) [pubmed]
  • Okada S, Fujisawa M, Sone T, Nakayama S, Nishiyama R, Takenaka M, Yamaoka S, Sakaida M, Kono K, Takahama M, Yamato TK, Fukuzawa H, Brennicke A, Ohyama K
    Construction of male and female PAC genomic libraries suitable for identification of Y-chromosome-specific clones from the liverwort, Marchantia polymorpha. Plant J. 24: 421-428 (2000)
  • Nishiyama R, Yamato TK, Miura K, Sakaida M, Okada S, Kono K, Takahama M, Sone T, Takenaka M, Fukuzawa H, Ohyama K
    Comparison of expressed sequence tags from male and female sexual organs of Marchantia polymorpha. DNA Res. 7:165-174 (2000)
  • Takenaka M, Yamaoka S, Hanajiri T, Shimizu-Ueda Y, Yamato TK, Fukuzawa H, Ohyama K
    Direct transformation and plant regeneration of the haploid liverwort, Marchantia polymorpha L. Transgenic Res. 9:179-185 (2000)
    [1999]

  • Kucho K, Ohyama K, Fukuzawa H
    CO2-responsive transcriptional regulation of CAH1 encoding carbonic anhydrase is mediated by enhancer and silencer regions in Chlamydomonas reinhardtii. Plant Physiol. 121:1329-38 (1999) [pubmed]
  • Asamizu E, Nakamura Y, Sato S, Fukuzawa H, Tabata S
    A large scale structural analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. DNA Res. 6:369-73 (1999) [pubmed]
  • Sone T, Fujisawa M, Takenaka M, Nakagawa S, Yamaoka S, Sakaida M, Nishiyama R, Yamato TK, Ohmido N, Fukui K, Fukuzawa H, Ohyama K
    Bryophyte 5S rDNA was inserted into 45S rDNA repeat units after the divergence from higher land plants. Plant Mol. Biol. 41:679-685 (1999)
  • Nishiyama R, Mizuno H, Okada S, Yamaguchi T, Takenaka M, Fukuzawa H, Ohyama K
    Two mRNA species encoding calcium-dependent protein kinases are differentially expressed in sexual organs of Marchantia polymorpha through alternative splicing. Plant Cell Physiol. 40:205-212 (1999)
  • Nagai J, Yamato TK, Sakaida M, Yoda H, Fukuzawa H, Ohyama K
    Expressed Sequence Tags from immature female sexual organ of a liverwort, Marchantia polymorpha. DNA Res. 6:1-11 (1999)
    [1998]

  • Fukuzawa H, Ishizaki K, Miura K, Matsueda S, Ino-ue T, Kucho K, Ohyama K
    Isolation and characterization of high CO2-requiring mutants from Chlamydomonas reinhardtii by gene tagging. Can. J. Bot. 76:1-6 (1998) [Link]
  • Akashi K, Takenaka M, Yamaoka S, Suyama Y, Fukuzawa H, Ohyama K
    Coexistence of nuclear DNA-encoded tRNAVal(AAC) and mitochondrial DNA-encoded tRNAVal(UAC) in mitochondria of a liverwort Marchantia polymorpha. Nucleic Acids Res. 26:2168-2172 (1998)
    [1997]

  • Kobayashi Y, Knoop V, Fukuzawa H, Ohyama K
    Interorganellar gene transfer in bryophytes: the functional nad7 gene is nuclear encoded in Marchantia polymorpha. Mol. Gen. Genet. 256:589-592 (1997)
  • Akashi K, Hirayama J, Takenaka M, Yamaoka S, Suyama Y, Fukuzawa H, Ohyama K
    Accumulation of nuclear-encoded tRNAThr(AGU) in mitochondria of the liverwort Marchantia polymorpha. Bioch. Biophys. Acta - Gene Structure and Expression 1350:262-266 (1997)
    [1996]

  • Akashi K, Sakurai K, Hirayama J, Fukuzawa H, Ohyama K
    Occurrence of nuclear-encoded tRNAIle in mitochondria of the liverwort Marchantia polymorpha. Current Genetics 30:181-185 (1996)
  • Katoh A, Lee KS, Fukuzawa H, Ohyama K, Ogawa T
    cemA homologue essential to CO2 transport in the cyanobacterium Synechocystis PCC6803. Proc Natl Acad Sci U S A. 93:4006-10 (1996)
    [pubmed]
    [1995]

  • Fukuzawa H, Uemura K, Ohyama K
    Understanding and utilization of inorganic carbon concentrating mechanism to improve plant CO2 fixation. Energy Conversion and Management 36:747-750 (1995)
    [Journal]
  • Takemura M, Nozato N, Oda K, Kobayashi Y, Fukuzawa H, Ohyama K
    Active transcription of the pseudogene for subunit 7 of the NADH dehydrogenase in Marchantia polymorpha mitochondria. Mol. Gen. Genet. 247:565-570 (1995)
    [1994]

  • Hiratsuka K, Wu X, Fukuzawa H, Chua NH
    Molecular dissection of GT-1 from Arabidopsis. Plant Cell 6:1805-1813 (1994)
  • Tachiki A, Dionisio-Sese ML, Fujiwara S, Tsuzuki M, Fukuzawa H, Miyachi S
    Effects of acetate on gene expression of external carbonic anhydrase in Chlamydomonas (Volvocales). Jpn. J. Phycol. 42:157-164 (1994)
    [1993]

  • Nozato N, Oda K, Yamato KT, Ohta E, Takemura M, Akashi K, Fukuzawa H, Ohyama K
    Cotranscriptional expression of mitochondrial genes for subunits of NADH dehydrogenase, nad5, nad4, nad2 in Marchantia polymorpha. Mol. Gen. Genet. 237:343-350 (1993)
    [1992]

  • Kinoshita T, Fukuzawa H, Shimada T, Saito T, Matsuda Y
    Primary structure and expression of a gamete lytic enzyme in Chlamydomonas reinhardtii: similarity of functional domains to matrix metalloproteases. Proc Natl Acad Sci U S A. 89:4693-7 (1992) [pubmed]
  • Tachiki A, Fukuzawa H, Miyachi S
    Characterization of carbonic anhydrase isozyme CA2, which is the CAH2 gene product, in Chlamydomonas reinhardtii. Biosci Biotechnol Biochem. 56:794-8. (1992) [pubmed]
  • Fukuzawa H, Suzuki E, Komukai Y, Miyachi S
    A gene homologous to chloroplast carbonic anhydrase (icfA) is essential to photosynthetic carbon dioxide fixation by Synechococcus PCC7942. Proc Natl Acad Sci U S A. 89:4437-41 (1992) [pubmed]
  • Ogura Y, Takemura M, Oda K, Yamato K, Ohta E, Fukuzawa H, Ohyama K
    Cloning and nucleotide sequence of a frxC-ORF469 gene cluster of Synechocystis PCC6803: conservation with liverwort chloroplast frxC-ORF465 and nif operon. Biosci Biotechnol Biochem. 56:788-93 (1992)
    [1991]

  • Suzuki E, Fukuzawa H, Miyachi S
    Identification of a genomic region that complements a temperature-sensitive, high CO2-requiring mutant of the cyanobacterium, Synechococcus sp. PCC7942. Mol Gen Genet. 226:401-8 (1991) [pubmed]
  • Fukuzawa H, Ishida S, Miyachi S
    cDNA cloning and gene expression of carbonic anhydrase in Chlamydomonas reinhardtii. Can. J. Bot. 69:1088-1096 (1991)
    [1990]

  • Fukuzawa H, Fujiwara S, Yamamoto Y, Dionisio-Sese ML, Miyachi S
    cDNA cloning, sequence, and expression of carbonic anhydrase in Chlamydomonas reinhardtii: regulation by environmental CO2 concentration. Proc Natl Acad Sci U S A. 87:4383-7 (1990) [pubmed]
  • Fujiwara S, Fukuzawa H, Tachiki A, Miyachi S
    Structure and differential expression of two genes encoding carbonic anhydrase in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 87:9779-83 (1990) [pubmed]
  • Fukuzawa H, Fujiwara S, Tachiki A, Miyachi S
    Nucleotide sequences of two genes CAH1 and CAH2 which encode carbonic anhydrase polypeptides in Chlamydomonas reinhardtii. Nucleic Acids Res. 18:6441-2 (1990) [pubmed]
  • Kamo T, Shimogawara K, Fukuzawa H, Muto S, Miyachi S
    Subunit constitution of carbonic anhydrase from Chlamydomonas reinhardtii. Eur.J.Biochem. 192:557-62 (1990) [pubmed]
  • Dionisio-Sese ML, Fukuzawa H, Miyachi S
    Light-Induced Carbonic Anhydrase Expression in Chlamydomonas reinhardtii. Plant Physiol. 94:1103-10 (1990) [pubmed]
    [1988]

  • Ohyama K, Fukuzawa H, Kohchi T, Sano T, Sano S, Shirai S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H
    Structure and organization of Marchantia polymorpha chloroplast genome. I. Cloning and gene identification. J.Mol.Biol. 203:281-298 (1988)
  • Umesono K, Inokuchi H, Shiki Y, Takeuchi M, Chang Z, Fukuzawa H, Kohchi T, Shirai H, Ohyama K, Ozeki H
    Structure and organization of Marchantia polymorpha chloroplast genome. II. Gene organization of the large single copy region from rps'12 to atpB. J.Mol.Biol. 203:299-331 (1988)
  • Fukuzawa H, Kohchi T, Sano T, Shirai H, Umesono K, Inokuchi H, Ozeki H, Ohyama K
    Structure and organization of Marchantia polymorpha chloroplast genome. III. Gene organization of the large single copy region from rbcL to trnI(CAU). J.Mol.Biol. 203:333-351 (1988)
  • Kohchi T, Shirai H, Fukuzawa H, Sano T, Komano T, Umesono K, Inokuchi H, Ozeki H, Ohyama K
    Structure and organization of Marchantia polymorpha chloroplast genome. IV. Inverted repeat and small single copy regions. J. Mol. Biol. 203: 353-372 (1988)
  • Ohyama K, Kohchi T, Fukuzawa H, Sano T, Umesono K, Ozeki H
    Gene organization and newly identified groups of genes of the chloroplast genome from a liverwort, Marchantia polymorpha. Photosynthesis Res. 16:7-22 (1988)
    [1987]

  • Fukuzawa H, Yoshida T, Kohchi T, Okumura T, Sawano Y, Ohyama K
    Splicing of group II introns in mRNAs coding for cytochrome b6 and subunit IV in the liverwort Marchantia polymorpha chloroplast genome. Exon specifying a region coding for two genes with the spacer region. FEBS Letters 220:61-66 (1987)
    [1986]

  • Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H
    Chloroplast gene organization deduced from complete sequence of liverwort Marchantia polymorpha chloroplast DNA. Nature 322:572-574 (1986)
  • Fukuzawa H, Kohchi K, Shirai H, Ohyama K, Umesono K, Inokuchi H, Ozeki H
    Coding sequences for chloroplast ribosomal protein S12 from the liverwort, Marchantia polymorpha are separated far apart on the different DNA strands. FEBS Letters 198:11-15 (1986)
  • Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H
    Complete nucleotide sequence of liverwort Marchantia polymorpha chloroplast DNA. Plant Mol.Biol.Rept. 4:148-175 (1986)
    [1985]

  • Yamano Y, Kohchi T, Fukuzawa H, Ohyama K, Komano T
    Nucleotide sequences of chloroplast 4.5S ribosomal RNA from a leafy liverwort, Jungermannia subulata, and a thalloid liverwort, Marchantia polymorpha. FEBS Letters 185: 203-207 (1985)
  • Fukuzawa H, Uchida Y, Yamano Y, Ohyama K, Komano T
    Molecular cloning of promoters functional in Escherichia coli from chloroplast DNA of a liverwort, Marchantia polymorpha. Agric.Biol.Chem. 49:2725-2731 (1985)
    [1984]

  • Tanaka A, Yamano Y, Fukuzawa H, Ohyama K, Komano T
    In vitro DNA synthesis by chloroplast isolated from Marchantia polymorpha L. cell suspension cultures. Agric.Biol.Chem. 48:1239-1244 (1984)
    [1983]

  • Ohyama K, Yamano Y, Fukuzawa H, Komano T, Yamagishi H, Fujimoto S, Sugiura M
    Physical mapping of chloroplast DNA from liverwort Marchantia polymorpha L. cell suspension cultures. Mol.Gen.Genet. 189:1-9 (1983)
    [1982]

  • Ohyama K, Wetter LR, Yamano Y, Fukuzawa H, Komano T
    A simple method for isolation of chloroplast DNA from Marchantia polymorpha L. cell suspension cultures. Agric.Biol.Chem. 46:237-242 (1982)